EXPLORATION AND IDENTIFICATION OF BIOLOGICAL CONTROL AGENT Trichoderma sp. IN-VITRO FROM BAMBOO PLANT

Abstract

Plant disease control using biological agents is an effort to control plant diseases without harming the environment. One of the biological agents that is widely used is the Trichoderma sp. It is known that Trichoderma sp. has high antagonism ability against microorganisms that cause plant diseases. In this study, Trichoderma sp. was explored from bamboo plantation soil samples from Surabaya city using a diagonal technique with five composite sample points in each plant area. Isolation was then carried out using the dilution method and continued with the spread plate technique on Potato Dextrose Agar media. In the isolation results, 2 fungal isolates were found that had macroscopic and microscopic characteristics consistent with Trichoderma sp. The macroscopic appearance found is that the hyphae colonies are white to dark green in color which have clear color gradation boundaries, and have rough-looking fibers. The microscopic appearance found was green fialid stem hyphae with an oval-shaped group of conidia.